腦垂體pituitary巨噬細胞清除Macrophage depletion方案
垂體（pituitary）是下丘腦-垂體-腎上腺 （hpa） 軸的重要樞紐。垂體激素分泌細胞 （hpc） 釋放多種激素，以在正常和壓力條件下調(diào)節(jié)基本身體功能。垂體內(nèi)分泌腺通過釋放促腎上腺皮質(zhì)激素 （acth） 來調(diào)節(jié)免疫系統(tǒng)，以響應(yīng)下丘腦的神經(jīng)元激活。然而，目前尚不清楚全身炎癥如何調(diào)節(jié)垂體hpc的轉(zhuǎn)錄組學(xué)特征。在這里，我們對小鼠垂體進行了單細胞rna測序（scrna-seq），發(fā)現(xiàn)在炎癥發(fā)生時，所有主要的垂體hpc都以細胞類型特異性方式產(chǎn)生強烈反應(yīng)，其中皮質(zhì)促物表現(xiàn)出強烈的反應(yīng)。全身炎癥還導(dǎo)致非典型生物活性分子的產(chǎn)生和釋放，包括皮質(zhì)激素的 nptx2，以調(diào)節(jié)免疫穩(wěn)態(tài)。同時，hpcs上調(diào)了趨化因子的基因表達，促進了hpcs與免疫細胞之間的通訊?？傊?，我們的研究揭示了垂體和免疫系統(tǒng)之間的廣泛相互作用，表明垂體在介導(dǎo)炎癥對身體生理學(xué)許多方面的影響方面發(fā)揮著多方面的作用。
macrophage depletion and virus injection in the pituitarymice were anesthetized with pentobarbital (80 mg/kg, i.p.) before surgery and then placed in a mouse stereotaxic instrument. injections were performed using a microsyringe pump and a micro4 controller (world precision instruments). for macrophage depletion, liposome-pbs or liposome-clodronate (liposoma,cp-005-005) was stereotaxically microinjected into the anterior pituitary (2.5 mm posterior from bregma, 0.4 mm lateral, 6 mm below pia). the liposomes were delivered to the target site at a rate of 60 nl/min for 500 nl per site. mice received saline or 0.5 mg/kg lps 18 h after liposome delivery and were killed 6 h after inflammation was established. for ccl2 expression and nptx2-ko, aav was delivered directly into the pituitary. the injection site, rate, and volume were the same as those used for the liposome injection. subsequent experiments were performed at least 3 weeks after virus injection.
(c) representative images showing iba1 (the marker of macrophage) expression in the pituitary from mice that received liposome-pbs (left) or liposome-clodronate (right) directly to the pituitary for 24 h. scale bar, 100 μm.
(d) serum concentrations of acth in saline- or lps-treated (0.5 mg/kg lps for 6 h) mice pretreated with liposome-pbs or liposome-clodronate (n = 4–7 mice).
(e) serum concentrations of corticosterone in lps-treated (0.5 mg/kg lps for 6 h) mice pretreated with liposome-pbs or liposome-clodronate (n = 6–7 mice).
什么是雙層保溫螺旋風(fēng)管
熱磁式氣體分析儀的原理
液壓絞車廠家的保養(yǎng)方法
JM-B-3S一體化振動變送器簡介
環(huán)氧樹脂砂漿適用于哪些方面
腦垂體pituitary巨噬細胞清除Macrophage depletion方案
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分析對于全鋼實驗臺有著哪些方面的要求呢？
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macrophage depletion and virus injection in the pituitarymice were anesthetized with pentobarbital (80 mg/kg, i.p.) before surgery and then placed in a mouse stereotaxic instrument. injections were performed using a microsyringe pump and a micro4 controller (world precision instruments). for macrophage depletion, liposome-pbs or liposome-clodronate (liposoma,cp-005-005) was stereotaxically microinjected into the anterior pituitary (2.5 mm posterior from bregma, 0.4 mm lateral, 6 mm below pia). the liposomes were delivered to the target site at a rate of 60 nl/min for 500 nl per site. mice received saline or 0.5 mg/kg lps 18 h after liposome delivery and were killed 6 h after inflammation was established. for ccl2 expression and nptx2-ko, aav was delivered directly into the pituitary. the injection site, rate, and volume were the same as those used for the liposome injection. subsequent experiments were performed at least 3 weeks after virus injection.
(c) representative images showing iba1 (the marker of macrophage) expression in the pituitary from mice that received liposome-pbs (left) or liposome-clodronate (right) directly to the pituitary for 24 h. scale bar, 100 μm.
(d) serum concentrations of acth in saline- or lps-treated (0.5 mg/kg lps for 6 h) mice pretreated with liposome-pbs or liposome-clodronate (n = 4–7 mice).
(e) serum concentrations of corticosterone in lps-treated (0.5 mg/kg lps for 6 h) mice pretreated with liposome-pbs or liposome-clodronate (n = 6–7 mice).
什么是雙層保溫螺旋風(fēng)管
熱磁式氣體分析儀的原理
液壓絞車廠家的保養(yǎng)方法
JM-B-3S一體化振動變送器簡介
環(huán)氧樹脂砂漿適用于哪些方面
腦垂體pituitary巨噬細胞清除Macrophage depletion方案
數(shù)控機床排屑機常見故障及處理方法
水果的好壞可以不用肉眼觀察-千代田CD-H240水果成熟度糖含量測試儀
廣安20KG砝碼，達州20KG砝碼，雅安20KG砝碼，巴中20KG砝碼
福建省粉塵濃度測量儀報警控制系統(tǒng)生產(chǎn)價格
紙箱打包機故障的排除和紙箱印刷機的保養(yǎng)
鋼管扣件試驗機用途概括及技術(shù)參數(shù)說明
分析對于全鋼實驗臺有著哪些方面的要求呢？
數(shù)控車銑復(fù)合機的保養(yǎng)要求
冷熱沖擊試驗箱“三室爭霸“
MAGPOWR離合器和制動器用TC-5功率放大器工作原理
室內(nèi)多參數(shù)環(huán)境檢測儀使用方法
立式加工中心油氣潤滑與油霧潤滑的不同
超聲波清洗機的維護與保養(yǎng)
GSJ雙曲面攪拌機的水力性能如何優(yōu)化？